Effects of Acremonium cellulase and heat-resistant lactic acid bacteria on lignocellulose degradation, fermentation quality, and microbial community structure of hybrid elephant grass silage in humid and hot areas

• DOI: 10.3389/fmicb.2022.1066753
• Publication Date: 2022-11-21T04:59:00Z
• Authors: Chen Chen; Yafen Xin; Xiaomei Li; Haoran Ni; Tairu Zeng; Zhaochang Du; Hao Guan; Yushan Wu; Wenyu Yang; Yimin Cai; Yanhong Yan
• Keywords: Silage

To better evaluate the effects of Acremonium cellulase (AC) and previously screened heat-resistant Lactobacillus plantarum 149 (LP149) on lignocellulose degradation, fermentation quality, microbial community during ensiling in humid hot areas, this study used a small-scale system to prepare hybrid elephant grass silage at 30 45°C, respectively. Compared control commercial inoculant (LP), addition AC or strain LP149 decreased contents neutral detergent fiber, acid cellulose increased glucose, fructose, sucrose fermentation. Furthermore, treatments altered communities' structure ensiling. treatment provided more substrate for fermentation, resulting an increase bacterial alpha diversity. abundance optimized compositions. In addition, had higher (P < 0.05) lactic acetic lower pH, butyric acid, NH3-N levels compared control. These results indicated that are promising additives can promote degradation improve quality areas.