Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems

• DOI: 10.3389/fmicb.2022.827228
• Publication Date: 2022-07-18T06:27:03Z
• Authors: Angela Cabezas; Gastón Azziz; Patricia Bovio-Wi...; Laura Fuentes; Lucía Braga; Jorge Wenzel; Silvia Sabaris; Silvana Tarlera; Claudia Etchebehere
• Keywords: Meltwater

Nitrogen cycle has been poorly investigated in Antarctic ecosystems. In particular, how extreme conditions of low temperature, dryness, and high radiation select the microorganisms involved is not yet understood. Denitrification an important step nitrogen which nitrate reduced stepwise to gases NO, N2O, N2. carried out by a wide group spread phylogenetic tree. The aim this work was isolate characterize denitrifying bacteria present different cold environments from Antarctica. Bacterial isolates were obtained lake, meltwater, sea, glacier ice, ornithogenic soil, penguin feces samples King George Island, Fildes peninsula Antarctic. Samples taken during deicing season five sampling campaigns. From all we able strains. A total 199 bacterial with capacity grow anaerobic mineral media reducing at 4°C obtained. characterization 16S rRNA gene sequence analysis showed predominance genus Pseudomonas, followed Janthinobacterium, Flavobacterium, Psychrobacter, Yersinia. Other minor genera detected Cryobacterium, Iodobacter, Kaistella, Carnobacterium. denitrify previously described for most related our many them genes suggesting presence new environment. Our demonstrates ubiquity denitrification Maritime Antarctica gives information linking temperature taxa unequivocal way.