Induction of Systemic Resistance in Maize and Antibiofilm Activity of Surfactin From Bacillus velezensis MS20

• DOI: 10.3389/fmicb.2022.879739
• Publication Date: 2022-05-09T16:18:55Z
• Authors: Shireen Adeeb Muj...; R. Z. Sayyed; Mohammad I. Mir; M. Y. Khan; Bee Hameeda; Mustfa F. Alkhanani; Shafiul Haque; Abdel Rahman Moha...; Péter Poczai
• Keywords: Surfactin; Lipopeptide

Surfactin lipopeptide is an eco-friendly microbially synthesized bioproduct that holds considerable potential in therapeutics (antibiofilm) as well agriculture (antifungal). In the present study, production of surfactin by a marine strain Bacillus velezensis MS20 was carried out, followed physico-chemical characterization, anti-biofilm activity, plant growth promotion, and quantitative Reverse Transcriptase—Polymerase Chain Reaction (q RT-PCR) studies. From results, it inferred found to produce biosurfactant (3,300 mg L –1 ) under optimized conditions. physicochemical characterization [Thin layer chromatography (TLC), Fourier Transform Infrared (FTIR) Spectroscopy, Liquid Chromatography/Mass Spectroscopy (LC/MS), Polymerase (PCR) amplification] revealed be surfactin. bio-assay scanning electron microscope (SEM) images, observed (MIC 50 μg Ml has appreciable bacterial aggregation against clinical pathogens Pseudomonas aeruginosa MTCC424, Escherichia coli MTCC43, Klebsiella pneumoniae MTCC9751, Methicillin resistant Staphylococcus aureus (MRSA) mycelial condensation property fungal phytopathogen Rhizoctonia solani . addition, q-RTPCR studies 8-fold upregulation (9.34 ± 0.11-fold) srf A-A gene compared controls. Further, treatment maize crop (infected with R. led defense enzymes. conclusion, concentration synergy carbon source inorganic/mineral salts can ameliorate yield and, application wise, antibiofilm antifungal activities. induced systemic resistance crop, which makes good candidate employed sustainable agricultural practices.