Enhancing epitope of PEDV spike protein

• DOI: 10.3389/fmicb.2022.933249
• Publication Date: 2022-07-22T15:28:54Z
• Authors: Techit Thavorasak; Monrat Chulanetra; Kittirat Glab-ampai; Kodchakorn Mahaso...; Nawannaporn Sae-lim; Karsidete Teerani...; Thaweesak Songserm; Rungrueang Yodshe...; Dachrit Nilubol; Wanpen Chaicumpa; Nitat Sookrung
• Keywords: Vero cell

Porcine epidemic diarrhea virus (PEDV) is the causative agent of a highly contagious enteric disease pigs characterized by diarrhea, vomiting, and severe dehydration. PEDV infects all ages, but neonatal during first week life are susceptible; mortality rates among newborn piglets may reach 80-100%. Thus, regarded as one most devastating pig viruses that cause huge economic damage to industries worldwide. Vaccination sows gilts at pre-fertilization or pre-farrowing stage good strategy for protection suckling against through acquisition lactating immunity. However, vaccination mother inducing high level virus-neutralizing antibodies complicated with unstandardized immunization protocol unreliable outcomes. Besides, vaccine also induce enhancing promote entry replication, so-called antibody-dependent enhancement (ADE), which aggravates upon new exposure. Recognition epitope induces production an existential necessity safe effective design. In this study, spike (S) protein was revealed time, using phage display technology mouse monoclonal antibody (mAbG3) bound S1 subunit S enhanced into permissive Vero cells lack Fc receptor. The phages displaying mAbG3-bound peptides derived from library panning mAbG3 matched several regions in S1-0 sub-domain subunit, indicating discontinuous (conformational). sequence linear S1-BCD sub-domains. Immunological assays verified mimotope results. Although molecular mechanism ADE caused via binding newly identified awaits investigation, data obtained study helpful useful designing subunit/DNA devoid epitope.