Physiological and transcriptomic analyses revealed gene networks involved in heightened resistance against tomato yellow leaf curl virus infection in salicylic acid and jasmonic acid treated tomato plants

• DOI: 10.3389/fmicb.2022.970139
• Publication Date: 2022-09-14T09:09:47Z
• Authors: Peng Wang; Sheng Sun; Kerang Liu; Rong Peng; Na Li; Bo Hu; Lumei Wang; Hehe Wang; Ahmed Jawaad Afzal; Xueqing Geng
• Keywords: Jasmonic acid; KEGG; Phenylpropanoid

Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus Geminiviridae family, causes disease tomato that significantly affects production worldwide. SA (salicylic acid), JA (jasmonic acid) or mimetic, COR (coronatine) applied exogenously resulted in improved resistance against TYLCV infection. When compared to mock treated leaves, pretreatment with three compounds followed by TYCLV stem infiltration also caused greater accumulation H2O2. We employed RNA-Seq (RNA sequencing) identify DEGs (differentially expressed genes) induced SA, JA, pre-treatments after Agro-inoculation tomato. To obtain functional information on these DEGs, we annotated genes using gene ontology (GO) and Kyoto encyclopedia genomes (KEGG) databases. Based our comparative analysis, differentially related cell wall metabolism, hormone signaling secondary metabolism pathways were analyzed compound samples. found levels affected SlNPR1 SlCOI1 silenced plants. Interestingly, samples, was hyper-activated plants which significant reduction viral titer, whereas SINPR1 silencing plants, there 19-fold increase load. Our results indicated had multiple impacts defense modulation at early stage These will help us better understand defenses invasion provide theoretical basis for breeding into commercial accessions.