Genomic diversity of resistant and virulent factors of Burkholderia pseudomallei clinical strains recovered from Guangdong using whole genome sequencing

• DOI: 10.3389/fmicb.2022.980525
• Publication Date: 2022-10-28T05:10:32Z
• Authors: Muhammad Shafiq; Bixia Ke; Xin Li; Mi Zeng; Yumeng Yuan; Dongmei He; Xiaoling Deng; Xiaoyang Jiao
• Keywords: Burkholderia pseudomallei; Melioidosis; Multilocus sequence typing; Phylogenetic diversity; Molecular Epidemiology

Background Burkholderia pseudomallei ( B. ) is a highly infectious agent and causes melioidosis, in both humans animals, which endemic Southeast Asia Northern Australia. Objectives This study aims to determine the molecular epidemiology, resistant determinants, genomic diversity of clinical isolates further elucidate phylogenetic evolutionary relationship strains with those other regions. Methods In this study, we obtained eight from Guangdong province 2018 2019. All were sequenced using Illumina NovaSeq platform. The draft genomes used find antibiotic-resistant genes (ARGs), virulence factors, gene mutations. Multilocus sequence typing (MLST) single nucleotide polymorphism (SNP) analysis performed characterize epidemiology strains. Results susceptible antibiotics commonly for melioidosis treatment. Class D beta-lactamases OXA-57 OXA-59, as well various mutation factors such amrA , amrB omp38 gyrA ceoB identified. MLST identified different types (STs): ST1774, ST1775, ST271, ST562, ST46, ST830, ST1325, ST10. Phylogenetic found that showed high genetic diversity. We also report 165 among responsible neurological disorders, pneumonia, skin lesions, abscesses. recovered antibiotics. However, exists isolates. surveillance, diagnosis, features varied geographical locations. These regional differences manifestations have implications practical management disease. Conclusion present reports identification