Antifungal susceptibility and molecular characteristics of Cryptococcus spp. based on whole-genome sequencing in Zhejiang Province, China

• DOI: 10.3389/fmicb.2022.991703
• Publication Date: 2022-11-17T04:29:54Z
• Authors: Junli Zhang; Zhengan Wang; Yan Chen; Zhihui Zhou; Qing Yang; Ying Fu; Feng Zhao; Xi Li; Qiong Chen; Li Fang; Yan Jiang; Yunsong Yu
• Keywords: Cryptococcus gattii; Multilocus sequence typing; Flucytosine

Cryptococcus spp. is a complex species that often causes cryptococcosis, which one of the most common opportunistic infections in adults living with HIV and has very high morbidity mortality rates. This study aimed to investigate antifungal susceptibility profiles epidemiological characteristics neoformans (CNSC) gattii (CGSC) Zhejiang Province, China. A total 177 CNSC 3 CGSC isolates were collected, was tested by FUNGUS verified an E-test. Moreover, multiple classification methods genomic analyses performed. The majority (96.11%) C. (formerly var. grubii) (ST5-VNI-A-α). Our highlights patients cryptococcosis non-HIV China, nearly half them did not have underlying diseases led immune insufficiency. Most this sensitive drugs. Two 5-flucytosine (5-FC)-resistant strains identified, FUR1 mutation detected 5-FC-resistant isolates. Typing based on whole-genome sequencing (WGS) showed better discrimination than achieved multilocus sequence typing (MLST) indicated clear population structure. phylogenetic analysis WGS included more information traditional methods.