Faecalibacterium prausnitzii prevents hepatic damage in a mouse model of NASH induced by a high-fructose high-fat diet

• DOI: 10.3389/fmicb.2023.1123547
• Publication Date: 2023-03-16T06:18:35Z
• Authors: Ji-Hee Shin; Yoonmi Lee; Eun-Ji Song; Dokyung Lee; Seo-Yul Jang; Hye Rim Byeon; Moon-Gi Hong; Sang-Nam Lee; Hyun-Jin Kim; Jae-Gu Seo; Dae Won Jun; Young-Do Nam
• Keywords: Faecalibacterium prausnitzii

Nonalcoholic steatohepatitis (NASH) is an advanced nonalcoholic fatty liver disease characterized by chronic inflammation and fibrosis. A dysbiosis of the gut microbiota has been associated with pathophysiology NASH, probiotics have proven helpful in its treatment prevention. Although both traditional next-generation potential to alleviate various diseases, studies that observe therapeutic effect on NASH are lacking. Therefore, we investigated whether a probiotic candidate, Faecalibacterium prausnitzii, contributed mitigation NASH.In this study, conducted 16S rRNA sequencing analyses patients healthy controls. To test F. prausnitzii could symptoms, isolated four strains (EB-FPDK3, EB-FPDK9, EB-FPDK11, EB-FPYYK1) from fecal samples collected individuals. Mice were maintained high-fructose high-fat diet for 16 weeks induce model received oral administration bacterial strains. Changes characteristic phenotypes assessed via glucose tolerance tests, biochemical assays, histological analyses.16S confirmed relative abundance reduced significantly compared controls (p < 0.05). In mice, supplementation improved homeostasis, prevented hepatic lipid accumulation, curbed damage fibrosis, restored damaged barrier functions, alleviated steatosis inflammation. Furthermore, real-time PCR assays documented regulated expression genes related these mice.Our therefore, confirms bacteria can symptoms. We propose contribute NASH.