Farm management practices and season dependent factors affect the microbial community and chemical profile of corn and grass-legume silages of farms in Ontario, Québec, and Northern New York

• DOI: 10.3389/fmicb.2023.1214915
• Publication Date: 2023-07-19T07:11:42Z
• Authors: Jesse Huffman; Pascal Drouin; Justin B. Renaud; Lysiane Dunière; Gisèle LaPointe
• Keywords: Silage

The effects of farm management practices and seasonal variation on the microbial community chemical composition corn grass-legume silage are largely understudied due to advantages controlled mini-silo experiments. This study aims investigate that some key factors (use an inoculant, region, bunker or tower silo) variations have from farms across Ontario, Quebec, New York. was either treated with a commercial inoculant (Lallemand Biotal Buchneri 500® Chr Hansen SiloSolve FC®) left untreated. bacterial communities were compared those raw bulk tank milk same determine if they similarly affected by variations. Family level analysis 16S rRNA V3-V4 gene amplicon community, ITS1 fungal NMR water soluble metabolome, mycotoxin LC-MS performed over two-year period. Chemical compounds associated use inoculants in higher inoculated (acetate, propane-1,2-diol, γ-aminobutyrate; p < 0.001) (propionate; = 0.011). However, there no significant difference relative abundance (RA) Lactobacillaceae type. Leuconostocaceae non-inoculated (p than silage. Tower silos had RA pH one used liquid manure other fertilizer type Clostridiaceae 0.045) rumen/fecal 0.006) bacteria all farms. Seasonal most families, however trends rarely visible both years. Few could be observed milk: two Moraxellaceae In using lower Staphylococcaceae milk.