Lead induced structural and functional damage and microbiota dysbiosis in the intestine of crucian carp (Carassius auratus)

• DOI: 10.3389/fmicb.2023.1239323
• Publication Date: 2023-09-04T09:16:52Z
• Authors: Haisu Liu; Hang Zhang; Qianxun Yu; Sanshan Zhang; Xiao Tu; Fenghong Zhuang; Shengli Fu
• Keywords: Crucian carp; Dysbiosis

Lead (Pb) is a hazardous pollutant in water environments that can cause significant damage to aquatic animals and humans. In this study, crucian carp ( Carassius auratus ) were exposed waterborne Pb for 96 h; then, histopathological analysis, quantitative qPCR 16S high-throughput sequencing performed explore the effects of on intestinal bioaccumulation, structural damage, oxidative stress, immune response, microbiota imbalance C. . After exposure, morphology was obviously damaged, including significantly increasing thickness wall number goblet cells reducing depth crypts. exposure reduced mRNA expressions Claudin-7 villin-1 while elevated level GST, GSH, CAT, IL-8, IL-10, IL-1 , TNF- α. Furthermore, rRNA analysis showed Shannon Simpson indices decreased at 48 h after abundance pathogenic bacteria (Erysipelotrichaceae, Weeksellaceae, Vibrionaceae) increased exposure. addition, correlation network found Proteobacteria negatively correlated with Firmicutes positively Bacteroidetes Functional prediction speculated change led PPAR signaling pathway peroxisome function intestine increased, system membrane transport decreased. Finally, canonical (CCA) there correlations between microbiota, morphology, antioxidant factors, factors Taken together, our results demonstrated flora dysbiosis, morphological disruption, injury are involved toxic structure carp. Meanwhile, rapidly bacteria, leading disorders, further aggravating function. These findings provide us basis link gut microbiome changes heavy metal toxicity, be used as biomarkers evaluation pollution future.