Compound Chinese medicine (F1) improves spleen deficiency diarrhea by protecting the intestinal mucosa and regulating the intestinal flora

• DOI: 10.3389/fmicb.2023.1292082
• Publication Date: 2024-01-16T15:53:42Z
• Authors: Kang Wang; Guanzong Li; Zhi Yang; Fumei Yang; Yulin Sun; Gang Duan; Wang Sun; Ke Zhou; Jun He; Feiyan Dai
• Keywords: Senna; Intestinal mucosa

Compound Chinese medicine (F1) is a traditional prescription in that commonly used to treat spleen deficiency diarrhea (SDD). It has demonstrated remarkable effectiveness clinical practice. However, the precise mechanism by which it exerts its antidiarrheal effect still unclear. This study aimed at investigating efficacy and of F1 on senna-induced secretory Senna was utilized induce development mouse model (SDD) order observe rate diarrhea, index, blood biochemistry, histopathological changes small intestine. Additionally, levels sodium hydrogen exchange protein 3 (NHE3) short-chain fatty acids (SCFAs) were determined using enzyme-linked immunosorbent assay (ELISA). The impact SDD models evaluated monitoring gut microbiota through 16S rRNA (V3-V4) sequencing. results F1, medicine, effectively increased body weight mice reduced incidence index. restored liver kidney function, infiltration inflammatory cells intestinal tissue, promoted growth villi. Furthermore, found enhance expression NHE3 SCFAs. also abundance Firmicutes Lactobacillus species, while decreasing Proteobacteria Shigella.