Transcriptomic analysis of cell envelope inhibition by prodigiosin in methicillin-resistant Staphylococcus aureus
Prodigiosin
Secondary metabolite
Cell envelope
DOI:
10.3389/fmicb.2024.1333526
Publication Date:
2024-01-22T04:46:41Z
AUTHORS (10)
ABSTRACT
Methicillin-resistant Staphylococcus aureus (MRSA) is a leading threat to public health as it resistant most currently available antibiotics. Prodigiosin secondary metabolite of microorganisms with broad-spectrum antibacterial activity. This study identified significant effect prodigiosin against MRSA minimum inhibitory concentration low 2.5 mg/L. The results scanning electron microscopy, crystal violet staining, and confocal laser microscopy indicated that inhibited biofilm formation in S. USA300, while also destroying the structure cell wall membrane, which was confirmed by transmission microscopy. At 1.25 mg/L, 76.24%, mg/L significantly reduced vitality cells biofilm. Furthermore, transcriptomic obtained at 1/8 MIC 235and 387 genes USA300 were up- downregulated, respectively. downregulated related two-component systems, including transcriptional regulator LytS, quorum sensing histidine kinases SrrB, NreA NreB, peptidoglycan biosynthesis enzymes (MurQ GlmU), iron-sulfur cluster repair protein ScdA, microbial surface components recognizing adaptive matrix molecules, well key arginine synthesis ArcC ArgF. upregulated mainly biosynthesis, systems vancomycin resistance-associated regulator, lipoteichoic acid proteins DltD DltB, 9 capsular polysaccharide proteins. elucidated molecular mechanisms through affects envelope from perspectives synthesis, membrane formation, providing new potential targets for development antimicrobials treatment MRSA.
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