Unraveling the important role of comammox Nitrospira to nitrification in the coastal aquaculture system

• DOI: 10.3389/fmicb.2024.1355859
• Publication Date: 2024-04-23T14:38:06Z
• Authors: Xueqin Yang; Yongjie Wu; Longfei Shu; Hang Gu; Fei Liu; Jijuan Ding; Jiaxiong Zeng; Cheng Wang; Zhili He; Meiying Xu; Feifei Liu; Xiafei Zheng; Bo Wu
• Keywords: Nitrospira

Increasing nitrogen (N) input to coastal ecosystems poses a serious environmental threat. It is important understand the responses and feedback of N removal microbial communities, particularly nitrifiers including newly recognized complete ammonia-oxidizers (comammox), improve aquaculture sustainability. In this study, we conducted holistic evaluation functional communities responsible for nitrification by quantifying sequencing key genes comammox Nitrospira-amoA, AOA-amoA, AOB-amoA Nitrospira-nxrB in fish ponds with different feeding levels evaluated contribution process through experiments mixing pure cultures. We found that higher dramatically increased N-related concentration, affecting nitrifying communities. Compared AOA AOB, Nitrospira NOB were more sensitive changes. Unexpectedly, detected an equivalent abundance AOB observed increase proportion clade A feeding. Furthermore, simplified network shift keystone species from fish-feeding ponds. Random forest analysis suggested community played critical role eutrophic (40–70 μM). Through additional experiment cultures, primary contributor at 200 μM ammonium. These results advance our understanding highlight importance driving systems.