Whole-genome sequencing of Sphingobium baderi SC-1 and identification of a crucial 3-phenoxybenzoic acid-degrading gene

• DOI: 10.3389/fmicb.2024.1361335
• Publication Date: 2024-04-05T04:43:43Z
• Authors: Qin Li; Qiao Zhou; Yuan Chen; Kaidi Hu; Montserrat Sarrà; Jianlong Li; Aiping Liu; Likou Zou; Shuliang Liu
• Keywords: Sphingomonas; Strain (injury); Degradation

As an efficient degradation strain, Sphingobium baderi SC-1 can breakdown 3-phenoxybenzoic acid (3-PBA) with high proficiency. To investigate the internal factors that regulate this process, we conducted whole-genome sequencing and successfully identified pivotal 3-PBA-degrading gene sca (1,230 bp). After was expressed in engineered bacteria, a remarkable efficiency observed, as 20 mg/L 3-PBA almost completely decomposed within 24 h. The phenol formed one of products. Notably, addition to their ability degrade 3-PBA, resting cells proficiently degraded 4′-HO-3-PBA 3′-HO-4-PBA. In conclusion, validated enzyme responsible for from Sphingomonas , providing crucial theoretical foundation further explorations on potential SC-1.