Genome-wide identification of resistance genes and response mechanism analysis of key gene knockout strain to catechol in Saccharomyces cerevisiae

• DOI: 10.3389/fmicb.2024.1364425
• Publication Date: 2024-02-21T04:30:21Z
• Authors: Hong Liao; Qian Li; Yulei Chen; Jiaye Tang; Borui Mou; Fujia Lu; Peng Feng; Wei Li; Jialian Li; Chun Fu; Wencong Long; Ximeng Xiao; Xuebing Han; Wenli Xin; Fengxuan Yang; Menggen Ma; Beidong Liu; Yaojun Yang; Hanyu Wang
• Keywords: Gene knockout

Engineering Saccharomyces cerevisiae for biodegradation and transformation of industrial toxic substances such as catechol (CA) has received widespread attention, but the low tolerance S. to CA limited its development. The exploration modification genes or pathways related in is an effective way further improve utilization efficiency CA. This study identified 36 associated with through genome-wide identification bioinformatics analysis ERG6 knockout strain ( Δ) most sensitive Based on omics Δ under stress, it was found that affects intrinsic component membrane pentose phosphate pathway. In addition, revealed 29 cell wall-membrane system were up-regulated by more than twice, NADPH NADP + increased 2.48 4.41 times respectively, spermidine spermine 2.85 2.14 times, Δ. Overall, response system, accumulation NADPH, well levels metabolites pathway are important findings improving resistance. provides a theoretical basis strains reducing damage caused ecological environment human health.