Heat-killed Akkermansia muciniphila ameliorates allergic airway inflammation in mice

• DOI: 10.3389/fmicb.2024.1386428
• Publication Date: 2024-05-09T04:51:46Z
• Authors: Seol Ah Yoon; Younggap Lim; Hye Rim Byeon; Jiyeon Jung; Seongho Ma; Moon-Gi Hong; Dohak Kim; Eun-Ji Song; Young-Do Nam; Jae-Gu Seo; Sang-Nam Lee
• Keywords: Akkermansia muciniphila; Goblet cell

Allergic asthma (AA) is a common inflammatory airway disease characterized by increased hyper-responsiveness (AHR), inflammation, and remodeling. Akkermansia muciniphila strictly anaerobic bacterium residing in the gut promising next-generation probiotic to improve metabolic syndrome. A recent study suggested beneficial effect of live A. on allergic inflammation (AAI) mice. However, whether heat-killed form can AAI requires further investigation. Mice sensitized challenged with house dust mites (HDM) develop AA hallmarks including cell infiltration, goblet hyperplasia, subepithelial collagen deposition lungs. These phenomena were reversed oral administration strain EB-AMDK19 (AMDK19-HK) isolated from feces healthy Koreans. Furthermore, AMDK19-HK diminished HDM-induced AHR inhaled methacholine, lung mast accumulation, serum HDM-specific IgE levels. It also led overall suppression IL-4, IL-13, eotaxin production bronchoalveolar lavage fluids, Il4 , Il5 Il13 Ccl17 gene expression tissues. Moreover, suppressed Th2-associated cytokine splenocytes HDM-sensitized mice vitro . Additionally, combination 16S rRNA sequencing short-chain fatty acid (SCFA) analysis cecal samples revealed that modulated relative abundance circulating SCFA-associated genera, positive correlation Lachnospiraceae_ NK4A136_group negative Lachnoclostridium significantly SCFA concentrations. Finally, improved intestinal mucosal barrier function. results suggest ameliorates suppressing Th2-mediated immune responses could have protective against development.