Characterization of Salmonella phage of the genus Kayfunavirus isolated from sewage infecting clinical strains of Salmonella enterica

• DOI: 10.3389/fmicb.2024.1391777
• Publication Date: 2024-06-03T04:29:26Z
• Authors: Ramya Juliet; Archana Loganathan; Ayyanraj Neeravi; Yamuna Devi Bakth...; Balaji Veeraraghavan; Prasanth Manohar; Ramesh Nachimuthu
• Keywords: Lytic cycle; Salmonella enterica; Phage therapy

The emergence of multi-drug resistance in Salmonella , causing food-borne infections, is a significant issue. With over 2,600 serovars sp., it crucial to identify specific solutions for each serovar. Phage therapy serves as an alternate treatment option. In this study, vB_SalP_792 phage was obtained from sewage, forming plaques eight out 13 tested clinical S. enterica isolates. Transmission electron microscopy (TEM) examination revealed T7-like morphotype. characterized by its stability, life cycle, antibiofilm, and lytic ability food sources. remains stable throughout range temperatures (−20 70°C), pH levels (3–11), chloroform ether. It also exhibited activity within MOIs 0.0001 100. cycle that 95% the phages attached their host 3 min, followed 5-min latent period, resulting 50 PFU/cell burst size. genome has dsDNA with length 37,281 bp GC content 51%. There are 42 coding sequences (CDS), 24 having putative functions no or virulence-related genes. significantly reduced bacterial load established biofilms egg whites. Thus, can serve effective biocontrol agent preventing infections food, potent against isolates sets successful candidate future vivo studies therapeutical application drug-resistant infections.