Nanopore-based targeted next-generation sequencing of tissue samples for tuberculosis diagnosis

• DOI: 10.3389/fmicb.2024.1403619
• Publication Date: 2024-07-03T05:18:00Z
• Authors: Weiwei Gao; Chen Yang; Tianzhen Wang; Yicheng Guo; Yi Zeng
• Keywords: Nanopore

Objective Diagnosing tuberculosis (TB) can be particularly challenging in the absence of sputum for pulmonary cases and extrapulmonary TB (EPTB). This study evaluated utility nanopore-based targeted next-generation sequencing (tNGS) diagnosing tissue samples, compared its efficacy with other established diagnostic methods. Methods A total 110 samples from clinical were examined. The sensitivity specificity tNGS benchmarked against a range existing approaches including hematoxylin eosin (HE) staining conjunction acid-fast bacilli (AFB) detection, HE combined PCR, paired immunohistochemistry (IHC) using anti-MPT64, Xpert Mycobacterium (MTB)/rifampicin (RIF) assay. Results 88.2 94.1%, respectively. respective sensitivities AFB, IHC MTB/RIF 30.1, 49.5, 47.3, 59.1%. specificities these methods 82.4, 88.2, 94.1, Analysis drug resistance based on results indicated that 10 93 patients (10.75%) had potential resistance. Conclusion Targeted achieved higher accuracy than methods, play crucial role rapid accurate diagnosis TB, drug-resistant TB.