Development of a colloidal gold immunochromatographic strip for the simultaneous detection of porcine epidemic diarrhea virus and transmissible gastroenteritis virus

• DOI: 10.3389/fmicb.2024.1418959
• Publication Date: 2024-06-19T05:06:46Z
• Authors: Jinzhu Zhou; Wei Wu; Dandan Wang; Wei Wang; Xinjian Chang; Yunchuan Li; Jizong Li; Baochao Fan; Junming Zhou; Rongli Guo; Xuejiao Zhu; Bin Li
• Keywords: Coronavirus; Gold standard (test)

In recent years, porcine diarrhea-associated viruses have caused significant economic losses globally. These present similar clinical symptoms, such as watery diarrhea, dehydration, and vomiting. Co-infections with epidemic diarrhea virus (PEDV) transmissible gastroenteritis (TGEV) are common. For the rapid on-site preliminary diagnosis on pig farms, this study aimed to develop a colloidal gold immunochromatography assay (GICA) strip for detection of PEDV TGEV simultaneously. The GICA kit showed that there was no cross-reactivity other five common viruses. With visual observation, lower limits were approximately 104 TCID50/mL TGEV, respectively. could be stored at 4°C or 25°C 12 months without affecting its efficacy. To validate strip, 121 samples tested. positive rates 42.9 9.9%, respectively, co-infection rate two 5.8% based duplex strip. Thus, established is rapid, specific, stable tool PEDV- TGEV-associated diarrhea.