The synergistic effects of humic acid, chitosan and Bacillus subtilis on tomato growth and against plant diseases

• DOI: 10.3389/fmicb.2025.1574765
• Publication Date: 2025-04-17T05:25:03Z
• Authors: Cunpu Qiu; Ying Bao; Dingding Lü; Mengyuan Yan; Guilong Li; Kai Liu; Shiping Wei; Meng Wu; Zhongpei Li

Understanding the effects of bio-antimicrobial materials on plant growth and against diseases relevant mechanisms are highly important for sustainable soil use safety production. This study explored impacts corresponding combined utilization humic acid, chitosan, Bacillus subtilis (B. subtilis) tomato occurrence through a greenhouse pot experiment. The height, fresh weight, disease index, rhizosphere microbial community, root exudates composition tomatoes were determined. With application chitosan B. (HBC), height weight plants significantly increased (p < 0.05), incidence was decreased by 45.1%. In HBC treatment, diversity fungal bacterial communities notably enhanced. relative abundances Bacillus, Gemmatimonas, Neobacillus, Acinetobacter, Humicola increased, while Sphingomonas, especially soil-borne pathogen Fusarium Ralstonia, 0.05). Besides exudates, content phenolic acids, which allelochemicals related to continuous cropping disorder, decreased. results cooccurrence network analysis indicated that Eicosanoids, Fatty acids conjugates, Flavonoid lycosides compounds in positively correlated with pathogenic bacteria, treatment. Results HBC's synergistic effect resistance is its regulation community exudates. promote development biological control technology highlight promising