The hsp70 new functions as a regulator of reproduction both female and male in Ophraella communa

• DOI: 10.3389/fmolb.2022.931525
• Publication Date: 2022-09-20T12:32:48Z
• Authors: Yan Zhang; Weihua Ma; Chao Ma; Qinglu Zhang; Zhenya Tian; Zhenqi Tian; Hongsong Chen; Jianying Guo; Fanghao Wan; Zhongshi Zhou
• Keywords: Male fertility

Heat shock proteins (Hsps) function as molecular chaperones that enable organisms to withstand stress and maintain normal life activities. In this study, we identified heat protein 70 (encoded by hsp70), which exhibits a higher expression in the mature male testis than unmature of Ophraella communa. Tissue profile revealed Ochsp70 levels males were highest testis, whereas those females head. Moreover, was found be significantly induced female bursa copulatrix after mating. Double-stranded RNA dsOchsp70 injected into performance interference, decreased within 20 d post-injection, no effect observed on level mating with dsOchsp70-injected males. However, significant downregulation fertility marked simultaneously. Furthermore, knockdown also led reduction fertility. Finally, comparative transcriptomic analysis glucose dehydrogenase insulin-like growth factor binding putative downstream targets Ochsp70. Overall, deduced is an indispensable gene potential O. communa, regulates reproduction.