Functional maturation of human iPSC-derived pyramidal neurons in vivo is dependent on proximity with the host tissue

• DOI: 10.3389/fncel.2023.1259712
• Publication Date: 2023-11-23T14:05:54Z
• Authors: Célia Raïs; Daniela Gaspar Sa...; Giulia Sansone; Stéphane Blanchard; Jean-Pierre Bourg...; Bernd Jagla; Baptiste Saudemont; Laurène Schlick; Stéphanie Pons; Uwe Maskos
• Keywords: Human brain

Human induced pluripotent stem cells (hiPSCs) have been used extensively in vitro to model early events neurodevelopment. Because of a number shortcomings, previous work has established potential use these vivo after transplantation into the mouse brain. Here, we describe systematic approach for analysis transplanted hiPSC-derived neurons and glial over time Using functional two-photon imaging GCaMP6f- expressing human neural cells, define quantify embryonic-like features their spontaneous activity. This is substantiated by detailed electron microscopy (EM) graft. We relate this synaptic development undergo up 7 months vivo. system can now be further genetic or experimental manipulation developing addressing neurodevelopmental diseases like schizophrenia Autism Spectrum Disorder.