Tandem mass tag-based quantitative proteomic analysis of effects of multiple sevoflurane exposures on the cerebral cortex of neonatal and adult mice

• DOI: 10.3389/fneur.2022.1056947
• Publication Date: 2022-12-13T06:30:40Z
• Authors: Jingyu Feng; Hua Lin; Yue Zhao; Yongyan Yang; Xiaoli Zhuang; Yang Yu; Yonghao Yu
• Keywords: Barnes maze

Sevoflurane is the most commonly used general anesthetic in pediatric surgery, but it has potential to be neurotoxic. Previous research found that long-term or multiple sevoflurane exposures could cause cognitive deficits newborn mice not adult mice, whereas short-term single inhalations had little effect on function at both ages. The mechanisms behind these effects, however, are unclear.In current study, 6- and 60-day-old C57bl groups were given 3% plus 60% oxygen for three consecutive days, each lasting 2 hours, while those control group only got oxygen. cortex tissues harvested 8th 62nd day. tandem mass tags (TMT)pro-based quantitative proteomics combined with liquid chromatography-tandem spectrometry (LC-MS/MS) analysis, Golgi staining, western blotting analysis applied analyze influences of anesthesia cerebral various Morris water maze (MWM) test was performed from postnatal day (P)30 P36 P84 P90 after treatment. affected spatial learning memory diminished dendritic spines primarily mature animals exhibited no significant alterations.A total 6247 proteins measured using methods TMTpro-labeled LC-MS/MS, 443 which associated age-dependent neurotoxic mechanism repeated anesthesia. Furthermore, revealed sevoflurane-induced brain damage may mediated by increasing levels protein expression CHGB, PTEN, MAP2c, decreasing level SOD2 expression.Our findings would help further mechanistic study neurotoxicity contribute seek effective protection developing under