Long-term iron supplementation combined with vitamin B6 enhances maximal oxygen uptake and promotes skeletal muscle-specific mitochondrial biogenesis in rats

• DOI: 10.3389/fnut.2023.1335187
• Publication Date: 2024-01-15T11:40:56Z
• Authors: Lei Zhou; Soroosh Mozaffari...; Attila Kolonics; Takuji Kawamura; Atsuko Koike; Johanna Kéringer; Yaodong Gu; Roman Karabanov; Zsolt Radák
• Keywords: MFN2; FIS1; SDHA; Muscle Atrophy

Introduction Iron is an essential micronutrient that plays a crucial role in various biological processes. Previous studies have shown iron supplementation related to exercise performance and endurance capacity improvements. However, the underlying mechanisms responsible for these effects are not well understood. Recent suggested beneficial impact of on mitochondrial function its ability rescue under adverse stress vitro rodents. Based current knowledge, our study aimed investigate whether changes resulting from associated with effect function. Methods In this study, we orally administered iron-based supplement rats 30 consecutive days at dosage 0.66 mg iron/kg body weight vitamin B6 0.46 mg/kg. Results Our findings reveal long-term supplementation, combination B6, led less gained increased VO 2 max rats. Besides, treatment substantially Complex I- II-driven ATP production intact mitochondria isolated gastrocnemius cerebellum. did change basal succinate-induced ROS cerebellum skeletal muscle. Furthermore, intervention significantly upregulated several muscle biogenesis metabolism-related biomarkers, including PGC-1α, SIRT1, NRF-2, SDHA, HSL, MTOR, LON-P. it affect muscular protein expression SIRT3, FNDC5, LDH, FIS1, MFN1, eNOS, nNOS. Interestingly, exert similar hippocampus Discussion conclusion, demonstrates increases max, possibly through positive regulating muscle-specific energy