Construction of Competitive Endogenous RNA Network and Verification of 3-Key LncRNA Signature Associated With Distant Metastasis and Poor Prognosis in Patients With Clear Cell Renal Cell Carcinoma

• DOI: 10.3389/fonc.2021.640150
• Publication Date: 2021-03-24T06:40:13Z
• Authors: Yang Su; Tianxiang Zhang; Jieqiong Tang; Li Zhang; Song Fan; Jun Zhou; Chaozhao Liang
• Keywords: Competing Endogenous RNA; Gene regulatory network

Clear cell renal carcinoma (ccRCC) is a common malignancy with high distant metastasis rate. Long non-coding RNAs (LncRNAs) are reported to be upregulated or downregulated in multiple cancers and play crucial role the of tumors prognosis. Therefore, purpose our study construct prognostic signature for ccRCC based on metastasis-related lncRNAs explore involved potential competitive endogenous RNA (ceRNA) network. The differentially expressed genes (DEGs) screened from database cancer genome atlas (TCGA) were used co-expression network identify module by weighted gene analysis (WGCNA). Key metastatic significance identified through rigorous screening, including survival analysis, correlation expression analyses stage, grade, metastasis, verified data set omnibus (GEO) profiling interactive (GEPIA). upstream miRNAs predicted via five online databases LncBase. Here, we constructed ceRNA key that significantly associated prognosis patients ccRCC. risk score model univariate, least absolute shrinkage selection operator (LASSO), multivariate Cox regression analyses, divided into high- low-risk groups according median score. Kaplan–Meier demonstrated mortality was higher high-risk group than group. Considering other clinical phenotype, indicated could function as an independent factor. Quantitative real-time (qRT)-PCR tissues cells high-expression level three lncRNAs. Gene enrichment (GSEA) revealed lncRNA mainly enriched autophagy- immune-related pathways, indicating autophagy immune functions may important In summary, independently predict ccRCC, related provided new sight mechanism promising therapeutic target