Increased AOC1 Expression Promotes Cancer Progression in Colorectal Cancer

• DOI: 10.3389/fonc.2021.657210
• Publication Date: 2021-05-05T06:20:09Z
• Authors: Fangyuan Liu; Weijun Ou; Wenbo Tang; Zhenyu Huang; Zhehui Zhu; Wenjun Ding; Jihong Fu; Yilian Zhu; Chenying Liu; Weimin Xu; Peng Du
• Keywords: Organoid; Tumor progression

Amine oxidase copper containing 1 (AOC1) is a gene whose biological function in colorectal cancer (CRC) has not been elucidated. Therefore, the purpose of this study was to investigate clinical significance AOC1 expression CRC and its cell lines.AOC1 levels were examined paired peritumoral tissues, distant liver metastatic tissues using quantitative real-time PCR, western blotting, immunohistochemistry staining. The log-rank test Cox regression model used analyze relationship between prognosis. Proliferation assays (Cell Counting Kit-8 colony formation assays), migration (Transwell wound healing assays) xenograft tumor nude mice performed assess role cells.AOC1 significantly increased human especially metastases, associated with worse In addition, had higher organoids than normal organoids, suggesting that it highly expressed epithelium. Functional analysis demonstrated knockdown inhibited proliferation cells by inducing EMT vitro. Xenograft showed xenografts growth vivo.High outcomes, an independent risk factor for poor prognosis, promoted aggressive phenotypes. expected become novel biomarker predicting prognosis patients effective therapeutic target practice.