Role of NRP1 in Bladder Cancer Pathogenesis and Progression

• DOI: 10.3389/fonc.2021.685980
• Publication Date: 2021-06-23T06:51:03Z
• Authors: Yang Dong; Wei-ming Ma; Zhen-duo Shi; Zhi-guo Zhang; Jia-he Zhou; Yang Li; Shao-qi Zhang; Kun Pang; Bi-bo Li; Wen-da Zhang; Tao Fan; Guang-yuan Zhu; Liang Xue; Rui Li; Ying Liu; Lin Hao; Cong-hui Han
• Keywords: Neuropilin

Bladder urothelial carcinoma (BC) is a fatal invasive malignancy and the most common of urinary system. In current study, we investigated function mechanisms Neuropilin-1 (NRP1), co-receptor for vascular endothelial growth factor, in BC pathogenesis progression. The expression NRP1 was evaluated using data extracted from GEO HPA databases examined cell lines. effect on proliferation, apoptosis, angiogenesis, migration, invasion cells were validated after knockdown. After identifying differentially expressed genes (DEGs) induced by silencing, GO/KEGG IPA ® bioinformatics analyses performed specific predicted pathways targets confirmed vitro. Additionally, co-expressed ceRNA network downloaded CCLE TCGA databases, respectively. High observed tissues cells. knockdown promoted apoptosis suppressed silencing activity MAPK signaling molecular cancer KEGG pathway analysis western blot also affected various biological functions, including antiviral response, immune cycle, proliferation migration cells, neovascularisation. Furthermore, main upstream molecule DEGs may be NUPR1 , downstream target essential regulation FOXP3 to activate DCBLD2 positively regulated PPAR significantly associated with low expression. We found that miR-204, miR-143, miR-145, miR-195 development. Our provide evidence aetiology first time demonstrated an association between NUPR1, FOXP3, DCBLD2. Specifically, downregulation contributes progression, which activation involved pathways. Therefore, serve as new therapeutic strategies treat other cancers.