Protein Tyrosine Kinase 7 Regulates EGFR/Akt Signaling Pathway and Correlates With Malignant Progression in Triple-Negative Breast Cancer

• DOI: 10.3389/fonc.2021.699889
• Publication Date: 2021-07-22T14:19:18Z
• Authors: Nai-Peng Cui; Shu Qiao; Shan Jiang; Jin-Lin Hu; Ting-Ting Wang; Wen-Wen Liu; Yan Qin; Ya-Nan Wang; Li-Shuang Zheng; Jin-Chao Zhang; Yong-Ping Ma; Bao-Ping Chen; Jian-Hong Shi
• Keywords: Triple-negative breast cancer

Triple-negative breast cancer (TNBC), the most aggressive subtype of cancer, is associated with high invasiveness, metastatic occurrence and poor prognosis. Protein tyrosine kinase 7 (PTK7) plays an important role in multiple cancers. However, PTK7 TNBC has not been well addressed. This study was performed to evaluate progression TNBC.Correlation expression clinicopathological parameters assessed using tissue microarray immunohistochemistry (IHC) staining 280 patients cancer. (MDA-MB-468, MDA-MB-436 MDA-MB-231) non-TNBC (MCF7 SK-BR-3) cell lines were examined immunoblotting assay. correlated genes invasive carcinoma analyzed cBioPortal datasets including 1,904 patients. overexpressed or knockdown (MDA-MB-468 MDA-MB-436) used analyze potential roles metastasis tumor progression. A bearing mouse model established further tumorigenicity vivo.PTK7 highly expressed correlates worse prognosis associates TNBC. Co-expression analysis gain- loss-of-function revealed that participates EGFR/Akt signaling regulation extracellular matrix organization migration COL1A1, FN1, WNT5B, MMP11, MMP14 SDC1. Gain- experiments suggested promotes proliferation lines. MDA-MB-468 demonstrated PTK7-deficiency inhibits vivo.This identified as a marker via pathway.