New panel of biomarkers to discriminate between amelanotic and melanotic metastatic melanoma

• DOI: 10.3389/fonc.2022.1061832
• Publication Date: 2023-01-26T08:58:02Z
• Authors: Ioana V. Militaru; Alina Adriana Rus; Cristian V.A. Mun...; Georgiana Manica; Stefana M. Petrescu
• Keywords: Amelanotic melanoma

Melanoma is a form of skin cancer that can rapidly invade distant organs. A distinctive feature melanomas their pigmentation status, as melanin present in most melanomas, whilst many metastatic tumors could become amelanotic. Besides the obvious malfunction key genes pathway, amelanotic bear characteristic molecular signature accounting for aggressivity. Using mass spectrometry-based proteomics we report here panel biomarkers aggressive melanoma differ from less invasive pigmented cells. The developed method allows label-free quantification proteins identified by LC-MS/MS analysis. We found set comprising AHNAK, MYOF, ANXA1, CAPN2, ASPH, EPHA2, THBS1, TGM2, ACTN4 along with involved cell adhesion/migration (integrins, PLEC, FSCN1, FN1) are highly expressed melanoma. Accompanying down regulation specific such tyrosinase and TYRP1, these type Interestingly, analysis hypoxia revealed abundance this normoxia was rather unaltered conditions. These therefore predict behaviour cells early stages tumor development thus serve prognostic. Applying algorithm to related databases including samples published independent laboratories/public confirm specificity newly signatures. Overall, begin unravel alterations how basic offers insights into assess clinical, pathological misdiagnosis differences between main subtypes