MECOM/PRDM3 and PRDM16 Serve as Prognostic-Related Biomarkers and Are Correlated With Immune Cell Infiltration in Lung Adenocarcinoma

• DOI: 10.3389/fonc.2022.772686
• Publication Date: 2022-01-31T04:42:22Z
• Authors: Meng Li; Hui Ren; Yanpeng Zhang; Na Liu; Meng Fan; Ke Wang; Tian Yang; Mingwei Chen; Puyu Shi
• Keywords: KEGG

Background The MDS1 and EVI1 complex locus (MECOM, also called PRDM3) PR domain containing 16 (PRDM16) are two highly related zinc finger transcription factors associated with many malignancies. However, the mechanisms of MECOM PRDM16 in prognosis tumor immune infiltration lung adenocarcinoma (LUAD) remain uncertain. Methods Cancer Genome Atlas (TCGA), Oncomine, UALCAN, GEPIA, TIMER databases were searched to determine relationship between expression PRDM16, clinicopathological features, infiltration, LUAD. Coexpressed genes investigated by CBioPortal, potential mechanism MECOM- PRDM16-related was elucidated GO KEGG analyses. STRING database utilized further construct protein-protein interaction network coexpressed genes, hub identified Cytoscape. Finally, qRT-PCR performed identify mRNA levels target Results downregulated LUAD ( p < 0.05), low age, gender, smoking duration, tissue subtype, poor stage, nodal metastasis status, TP53 mutation, 0.05). found be correlated a variety cell subsets their markers. analysis showed that both them mainly enriched cycle, cellular senescence, DNA replication, p53 signaling pathway. Importantly, decreased clinical samples qRT-PCR. Conclusion may serve as prognostic biomarkers which govern recruitment