Choline Kinase Alpha2 Promotes Lipid Droplet Lipolysis in Non-Small-Cell Lung Carcinoma

• DOI: 10.3389/fonc.2022.848483
• Publication Date: 2022-04-07T07:08:02Z
• Authors: Rongxuan Zhu; Yannan Yang; Fei Shao; Juhong Wang; Yibo Gao; Jie He; Zhimin Lu
• Keywords: Lipid droplet

Background Rapid tumor growth inevitably results in energy stress, including deficiency of glutamine, a critical amino acid for cell proliferation. However, whether glutamine allows cells to use lipid droplets as an resource and the mechanism underlying this potential regulation remain unclear. Methods We purified from H322 H358 human non-small-cell lung cancer (NSCLC) under deprivation conditions performed immunoblotting determine binding choline kinase (CHK) α2 droplets. Immunofluorescence was used quantify droplet numbers sizes. Immunoprecipitation were examine AMPK activation CHKα2 phosphorylation. Cellular fatty levels, mitochondrial acetyl coenzyme A ATP production, apoptosis proliferation measured. Immunohistochemical analyses expression levels ACC pS79 pS279 specimens NSCLC patients. The prognostic value assessed using Kaplan-Meier method Cox regression models. Results Glutamine induces AMPK-mediated S279 phosphorylation, which promotes droplets, resulting recruitment cytosolic lipase ATGL autophagosomes subsequent lipolysis sustain survival In addition, CHKα much higher than their adjacent normal tissues positively correlated with each other. Notably, alone associated poor prognosis patients, combined values both phosphorylation worse Conclusion plays role NSCLC. or combination can be markers