Comprehensive Analyses of miRNAs Revealed miR-92b-3p, miR-182-5p and miR-183-5p as Potential Novel Biomarkers in Melanoma-Derived Extracellular Vesicles

• DOI: 10.3389/fonc.2022.935816
• Publication Date: 2022-07-08T21:41:42Z
• Authors: Dennis Gerloff; Stefanie Kewitz-H...; Gerd Hause; Jovine Ehrenreich; Linda Golle; Tim Kingreen; Cord Sunderkötter
• Keywords: Extracellular Vesicles; Extracellular vesicles; Exosome

Extracellular vesicles (EVs) are important mediators in the intercellular communication, influencing function and phenotype of different cell types within tumor micro-milieu thus promote progression. Since EVs safely transport packages proteins, lipids also nucleic acids such as miRNAs, their cargo can serve diagnostic prognostic markers. Therefore, aim this study was to investigate EV embedded miRNAs specific for melanoma, which could potential biomarkers. In contrast previous studies, we not only analysed from EVs, but included miRNA profiles EV-secreting cells identify candidates suitable While characterization derived normal melanocytes melanoma showed largely comparable properties with regard size distribution expression protein markers, NGS analyses yielded marked differences several miRNAs. load human epidermal (NHEMs) were very similar, they highly secreting cells. By comprehensive analyses, six identified be enriched both cell-derived EVs. Of those, accumulation miR-92b-3p, miR-182-5p miR-183-5p validated vitro. functional network generation pathway enrichment analysis revealed an association entities signaling pathways contributing Furthermore, found that serum patients. Our results support hypothesis or liquid biopsy markers melanoma. We EV-derived those elevated serum-derived patients metastatic healthy subjects.