Characterizing microbial communities and their correlation with genetic mutations in early-stage lung adenocarcinoma: implications for disease progression and therapeutic targets

• DOI: 10.3389/fonc.2024.1498524
• Publication Date: 2025-01-07T05:35:17Z
• Authors: Hao-Shuai Yang; Jin Zhang; Hong-Xiang Feng; Fei Qi; Fan-Jia Kong; Wei-Jie Zhu; Chao-Yang Liang; Zhen-Rong Zhang
• Keywords: Oncology; PTPRZ1; Neoplasms. Tumors. Oncology. Including cancer and carcinogens; next-generation sequencing; lung adenocarcinoma; therapeutic targets; tumor microbiome; RC254-282

Lung adenocarcinoma (LUAD), the most prevalent form of lung cancer. The transition from in situ (AIS), and minimally invasive (MIA) to (IAC) is not fully understood. Intratumoral microbiota may play a role LUAD progression, but comprehensive stage-wise analysis lacking. Tumor bronchoalveolar lavage fluid (BALF) samples patients with AIS/MIA or IAC were collected for next-generation sequencing characterize microbial diversity composition. DNA extraction involved lysing nuclease protease, followed by homogenization elution. Sequencing libraries prepared sequenced on Illumina platform. Whole exome was performed identify somatic mutations genetic variants. Bioinformatics analysis, including taxonomic annotation Kraken2 de novo assembly MEGAHIT, conducted process metagenomic data. Correlation link species mutated genes using custom R scripts. Metagenomic revealed distinct profile compared AIS/MIA, increased abundance Bacteroidetes Firmicutes group. Bosea sp. Microbacterium paludicola, less abundant IAC, suggesting potential protective early-stage disease. Conversely, Mycolicibacterium more indicating possible contribution disease progression. Genetic identified PTPRZ1 strongly correlating composition, mechanistic between alterations LUAD. This study characterizes communities various stages LUAD, revealing links mutations. unique suggests its progression as therapeutic target.