Neuroprotective Effect of Astragaloside IV on Cerebral Ischemia/Reperfusion Injury Rats Through Sirt1/Mapt Pathway

• DOI: 10.3389/fphar.2021.639898
• Publication Date: 2021-03-26T06:14:54Z
• Authors: Yi-Hua Shi; Xi-Le Zhang; Peng-Jie Ying; Zi-Qian Wu; Le-Le Lin; Wei Chen; Guo-Qing Zheng; Wen-Zong Zhu
• Keywords: Stroke; Tau protein; Edaravone

Background: Ischemic stroke is a common disease with poor prognosis, which has become one of the leading causes morbidity and mortality worldwide. Astragaloside IV (AS-IV) main bioactive ingredient Astragali Radix (which been used for ischemic thousands years) found to have multiple bioactivities in nervous system. In present study, we aimed explore neuroprotective effects AS-IV rats cerebral ischemia/reperfusion (CIR) injury targeting Sirt1/Mapt pathway. Methods: Sprague–Dawley (male, 250–280 g) were randomly divided into Sham group, middle artery occlusion/reperfusion (MCAO/R) MCAO/R + EX527 (SIRT1-specific inhibitor) group. Each group was further assigned several subgroups according time (6 h, 1 d, 3 7 days). The CIR induced by MCAO surgery accordance modified Zea Longa criteria. Modified Neurological Severity Scores (mNSS) evaluate neurological deficits; TTC (2,3,5-triphenyltetrazolium chloride) staining detect infarction area; Western Blot assess protein levels SIRT1, acetylated MAPT (ac-MAPT), phosphorylated ( p -MAPT), total (t-MAPT); Real-time Quantitative Polymerase Chain Reaction (qRT-PCR) detection Sirt1 Mapt transcriptions. Results: Compared can significantly improve dysfunction < 0.05), reduce area raise expression SIRT1 alleviate abnormal hyperacetylation hyperphosphorylation 0.05). While compared showed higher mNSS scores more severe lower 0.01), ac-MAPT -MAPT Conclusion: deficit after area, exerts probably through