Excessive Intake of Longan Arillus Alters gut Homeostasis and Aggravates Colitis in Mice

• DOI: 10.3389/fphar.2021.640417
• Publication Date: 2021-03-26T06:20:54Z
• Authors: Huimin Huang; Mingxing Li; Yi Wang; Xiaoxiao Wu; Jing Shen; Zhangang Xiao; Yueshui Zhao; Fukuan Du; Yu Chen; Zhigui Wu; Huijiao Ji; Chunyuan Zhang; Jing Li; Qinglian Wen; Parham Jabbarzade...; Chi Hin Cho; Shengpeng Wang; Yitao Wang; Yisheng He; Xu Wu
• Keywords: longan; Pharmacology; 0301 basic medicine; 2. Zero hunger; free sugar; 03 medical and health sciences; gut microbiota; inflammatory bowel disease; Therapeutics. Pharmacology; RM1-950; short-chain fatty acid; 3. Good health

Background: Longan is the fruit of Dimocarpus longan Lour. and arillus has long been used in traditional Chinese medicine possessing various health benefits. However, excessive intake found daily life to cause “ shanghuo ” syndrome. Shanghuo linked increased disease susceptibility. The present study thus aimed investigate toxicological outcomes after treatment. Methods: extract at a normal dosage 4 g/kg two excess dosages 8 16 was orally administered C57BL/6J mice for weeks or with DSS-induced colitis. Mouse gut microbiome were analyzed by 16S rRNA sequencing. Short chain fatty acid (SCFA) contents colonic measured GC-MS. Colon tissue histopathological observation H E staining, detection protein expression western blot, analysis gene qPCR, apoptotic cells TUNEL assay. ELISA biochemical serum. Results: In mice, repeated doses, but not dose, infiltration inflammatory cells, elevated serum levels TNF-α IL-6 reduced production SCFAs. colitic did promote colitis while (8 g/kg) aggravated showing inflammation, more serious histological abnormalities, permeability, epithelia injury when compared DSS alone. Excessive induced significant reduction microbial diversity accompanied alterations DSS-associated bacteria including increase Proteobacteria phylum genera Bacteroides , Akkermansia Turicibacter Escherchia-Shigella decrease norank_f__Muribaculaceae . changed compositions decreased SCFAs supplemented DSS. colon tightly correlated altered communities production. Conclusion: disturbs homeostasis aggravates via promoting inflammation altering microbe associated metabolism mice. Our findings warrant rational consumption as dietary supplement herbal medicine.