(+)-Dehydrovomifoliol Alleviates Oleic Acid-Induced Lipid Accumulation in HepG2 Cells via the PPARα–FGF21 Pathway

• DOI: 10.3389/fphar.2021.750147
• Publication Date: 2021-11-19T07:47:19Z
• Authors: Yiyuan Xi; Jujia Zheng; Wei Xie; Xiangwei Xu; Namki Cho; Xudong Zhou; Xiaomin Yu
• Keywords: Lipid Accumulation; FGF21

An overload of hepatic fatty acids, such as oleic acid is a key trigger non-alcoholic liver disease (NAFLD). Here, we investigated whether Artemisia frigida , valuable traditional medicine used to treat various diseases, could mitigate OA-induced lipid accumulation in HepG2 cells. Then, identify the active substances A. phytochemistry investigation was conducted using bioassay-guided isolation method. Consequently, one terpene ( 1 ) and flavone 2 were identified. Compound ((+)-dehydrovomifoliol) exhibited potent effects against cells, without causing cyto-toxicity. Notably, treatment with (+)-dehydrovomifoliol decreased expression levels three genes related lipogenesis SREBP1, ACC, FASN increased those oxidation PPARα, ACOX1, FGF21 ). In addition, similar results observed for protein levels. The partially reversed by PPARα antagonist GW6471, indicating important role PPARα–FGF21 axis (+)-dehydrovomifoliol. Based on its signaling via axis, isolated from be useful early lead compound developing new drugs NAFLD prevention.