Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide

• DOI: 10.3389/fphar.2021.761908
• Publication Date: 2021-12-31T04:38:56Z
• Authors: Shu-Jun Dong; Xin-Yue Gao; Ming-Xin Pei; Ting Luo; Dong Fan; Yan-Ling Chen; Jun-Feng Jin; Xiao-Duo Zhao
• Keywords: Iopromide; Renal Injury

With the increasing application of medical imaging contrast materials, contrast-induced nephropathy (CIN) has become third major cause iatrogenic renal insufficiency. CIN is defined as an absolute increase in serum creatinine levels at least 0.50 mg/dl or >25% from baseline after exposure to contrast. In this study, protective effects salvianolic acid B (Sal B) were detected human tubular epithelial cells (HK-2) exposed iopromide. The results showed that different concentrations Sal counteract loss cell viability induced by iopromide, and reduce apoptosis, reactive oxygen species (ROS) levels, endoplasmic reticulum stress (ERS)-related apoptosis-related proteins such p-IRE-1α, p-eIF-2α/eIF-2α, p-JNK, CHOP, Bax/Bcl-2, cleaved caspase-3. addition, a concentration 100 μmol/L inhibited ERS reduced damage similar extent inhibitor 4-PBA. Importantly, treatment with could abolish injury agonist tunicamycin, mitochondrial membrane potential, well significantly reducing ROS expression cleaved-caspase-3, GRP78, p-eIF2α, CHOP. These suggested effect against HK-2 iopromide may be related inhibition ERS.