Hypoxia-Induced Upregulation of lncRNA ELFN1-AS1 Promotes Colon Cancer Growth and Metastasis Through Targeting TRIM14 via Sponging miR-191-5p

• DOI: 10.3389/fphar.2022.806682
• Publication Date: 2022-05-16T07:06:51Z
• Authors: Xu Jing; Lutao Du; Shuang Shi; Aijun Niu; Jing Wu; Yunshan Wang; Chuanxin Wang
• Keywords: Competing Endogenous RNA

Hypoxia is identified as one of the microenvironmental features most solid tumors and involved in tumor progression. In present research, we demonstrate that lncRNA extracellular leucine rich repeat fibronectin type III domain-containing 1-antisense RNA 1 (ELFN1-AS1) upregulated by hypoxia colon cancer cells. Knockdown ELFN1-AS1 hypoxic cells can reduce cell proliferation restore invasion to non-hypoxic levels. Fluorescence situ hybridization results show distributed cytoplasm cells, so further analyze potential targets for a competing endogenous (ceRNA). MiR-191-5p contains binding sequence with downregulated Then, there site between miR-191-5p 3' untranslated region tripartite motif TRIM 14 (TRIM14). The expression TRIM14 inhibited siRNA or mimics LoVo HT29 treatment inhibitor knockdown significantly increase ability. Overexpression miR-191-5p-mimic-treated rescue inhibition caused mimics. conclusion, operates downstream target hypoxia, promotes invasion, inhibits apoptosis through upregulating sponging Our enrich our understanding progression provide clinical cancer.