Puerarin inhibited oxidative stress and alleviated cerebral ischemia-reperfusion injury through PI3K/Akt/Nrf2 signaling pathway

• DOI: 10.3389/fphar.2023.1134380
• Publication Date: 2023-05-22T04:55:14Z
• Authors: Qianqian Zhang; Min Yao; Jiajia Qi; Rui Song; Lei Wang; Jiacheng Li; Xian Zhou; Dennis Chang; Qi Huang; Lili Li; Ning Wang
• Keywords: NeuN

Introduction: Puerarin (PUE) is a natural compound isolated from Puerariae Lobatae Radix, which has neuroprotective effect on IS. We explored the therapeutic and underlying mechanism of PUE cerebral I/R injury by inhibiting oxidative stress related to PI3K/Akt/Nrf2 pathway in vitro vivo. Methods: The middle artery occlusion reperfusion (MCAO/R) rats oxygen-glucose deprivation (OGD/R) were selected as models, respectively. was observed using triphenyl tetrazolium hematoxylin-eosin staining. Tunel-NeuN staining Nissl quantify hippocampal apoptosis. reactive oxygen species (ROS) level detected flow cytometry immunofluorescence. Biochemical method detect levels. protein expression Western blotting. Finally, co-immunoprecipitation used study molecular interaction between Keap1 Nrf2. Results:In vivo studies showed that improved neurological deficits rats, well decreased stress. Immunofluorescence indicated release ROS can be inhibited PUE. In addition, blotting results promoted phosphorylation PI3K Akt, enabled Nrf2 enter nucleus, further activated downstream antioxidant enzymes such HO-1. combination with inhibitor LY294002 reversed these results. Nrf2-Keap1 complex dissociation. Discussion: Taken together, activate via PI3K/Akt promote enzyme expression, could ameliorate stress, against I/R-induced Neuron injury.