Xiaoxuming decoction cutting formula reduces LPS-stimulated inflammation in BV-2 cells by regulating miR-9-5p in microglia exosomes

• DOI: 10.3389/fphar.2023.1183612
• Publication Date: 2023-05-17T04:32:46Z
• Authors: Menglei Wang; Yuting Yang; Yanlei Guo; Ruirong Tan; Yanmei Sheng; Huawei Chui; Ping Chen; Hua Luo; Zhujun Ying; Li Li; Jin Zeng; Junning Zhao
• Keywords: Stroke; Decoction; Inflammatory response

The Background: Stroke is one of the leading causes morbidity and mortality, inflammatory mechanism plays a crucial role in stroke-related brain injury post-ischemic tissue damage. Xiaoxuming decoction (XXMD) first prescription for treatment “zhongfeng” (a broad concept referring to stroke) Tang Song Dynasties China has significant position history stroke treatment. Through study ancient medical records modern clinical evidence, it evident that XXMD efficacy its sequelae, pharmacological may be related post-stroke inflammation. However, contains 12 medicinal herbs with complex composition, therefore, simplified version XXMD, called cutting (XXMD-C), was derived based on anti-inflammatory effects individual herbs. Therefore, necessary explore confirm XXMD-C. Aim study: Based previous experiments our research group, found both XXMD-C have LPS-induced microglia, better effect. Since miRNAs exosomes also participate occurrence development cardiovascular diseases, traditional Chinese medicine can regulate exosomal through intervention, this aims inflammation transcriptome sequencing, providing basis application Materials methods: extracted using water filtered 0.22 μm membrane filter. main chemical components were rapidly qualitatively analyzed ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Cell viability determined CCK-8 assay, an BV-2 cell model established. expression cytokines detected ELISA Western blot (WB). Extracellular vesicles ultracentrifugation, identified transmission electron microscopy (TEM), nanoparticle tracking analysis, WB. Differential screened smallRNA-seq validated RT-PCR blot. Results: UPLC-Q-TOF-MS analysis revealed representative including ephedrine, pseudoephedrine, cinnamaldehyde, baicalin, baicalein, wogonin, ginsenoside Rg1 results WB assays showed had therapeutic effect cells. TEM, demonstrated successful extraction extracellular high-speed centrifugation. miRNA by miR-9-5p, which Inhibition miR-9-5p downregulate factors IL-1β, IL-6, iNOS, TNF-α. Conclusion: anti-neuroinflammatory effects. sequencing vesicles, as key treating neuroinflammation, vivo deserves further investigation.