Antibacterial activity of isopropoxy benzene guanidine against Riemerella anatipestifer

• DOI: 10.3389/fphar.2024.1347250
• Publication Date: 2024-02-02T10:05:43Z
• Authors: Yixing Lu; Weimei Qiao; Yaqian Xue; Xiaoxin Hong; Yuhang Jin; Jie Li; Xianfeng Peng; Dongping Zeng; Zhenling Zeng
• Keywords: Guanidine

Introduction: Riemerella anatipestifer (R. anatipestifer) is an important pathogen in waterfowl, leading to substantial economic losses. In recent years, there has been a notable escalation the drug resistance rate of R. anatipestifer. Consequently, imperative need expedite development novel antibacterial medications effectively manage infection caused by . Methods: This study investigated vitro and vivo activities substituted benzene guanidine analog, namely, isopropoxy (IBG), against using microdilution method, time-killing curve, pericarditis model. The possible mechanisms these were explored. Results Discussion: minimal inhibitory concentration (MIC) range IBG for was 0.5–2 μg/mL. Time-killing curves showed concentration-dependent effect. alone or combination with gentamicin significantly reduced bacterial load Serial-passage mutagenicity assays low probability developing resistance. Mechanistic studies suggested that induced membrane damage binding phosphatidylglycerol cardiolipin, imbalance potential transmembrane proton gradient, as well decreased intracellular adenosine triphosphate. summary, controlling infections.