Targeted lipidomics analysis of possible molecular mechanisms of lipid changes in temporal lobe epilepsy models

• DOI: 10.3389/fphar.2024.1531524
• Publication Date: 2025-01-09T06:10:15Z
• Authors: Huaiyu Sun; Xuewei Li; Zhiqing Chen; Hongmei Meng
• Keywords: Pharmacology; epilepsy; therapeutic target; Therapeutics. Pharmacology; RM1-950; temporal lobe epilepsy; molecular mechanism; targeted lipidomics analysis

Lipids are vital biomolecules involved in the formation of various biofilms. Seizures can cause changes lipid metabolism brain. In-depth studies at multiple levels urgently needed to elucidate composition, distribution, and metabolic pathways brain after seizure. In this research, a cutting-edge targeted quantitative lipidomics study was conducted on hippocampal tissues six rats with temporal lobe epilepsy normal rats. Accurate quantification based linear equations calculated using an internal standard. The lipids were quantitatively qualitatively analyzed by ultra-high performance liquid chromatography (UPLC) mass spectrometry (MS). A total 21 classes identified. Among them, most abundant triacylglycerol (TG), phosphatidyl ethanolamine (PE-P), fatty acids (FA). Cholesteryl ester (ChE) exhibits considerable difference between epileptic samples. ChE found be significantly upregulated lipid, while FA observed downregulated lipid. Based absolute analysis rat specimens, contents change trends different observed. Upregulation dihydroceramide (DHCer) observed, distribution elucidated causes possible molecular mechanisms accumulation epilepsy. results methods described provide comprehensive new therapeutic target for treatment