TOR as a Regulatory Target in Rhipicephalus microplus Embryogenesis

• DOI: 10.3389/fphys.2019.00965
• Publication Date: 2019-07-31T04:36:58Z
• Authors: Camila Waltero; Leonardo Araujo d...; Thayná Alonso; Rodrigo Nunes-da-...; Itabajara da Silv...; Carlos Logullo
• Keywords: TOR signaling; Transcription

Embryogenesis is a metabolically intensive process carried out under tightly controlled conditions. The insulin signaling pathway regulates glucose homeostasis and essential for reproduction in metazoan model species. Three key targets are part of this pathway: protein kinase B (PKB, or AKT), glycogen synthase 3 (GSK-3), target rapamycin (TOR). While the role AKT GSK-3 has been investigated during tick embryonic development, TOR remains unknown. In study, two other downstream effectors, namely S6 (S6K) eukaryotic translation initiation factor 4E-binding 1 (4E-BP1), were vitro studies using cell line BME26. First, we show that exogenous can stimulate transcription. Second, chemical inhibition led to decrease BME26 viability, loss membrane integrity, downregulation S6K 4E-BP1 Conversely, treating cells with inhibitors did not affect transcription, showing specifically required activate its targets. To address reproduction, vivo performed. Analysis relative transcription different stages development showed levels TOR, maternal deposition transcripts. Injection double-stranded RNA (dsRNA) into partially fed females slight delay oviposition, an atypical egg external morphology, decreased vitellin content eggs, larval hatching. Taken together, our data important acts as regulatory Rhipicephalus microplus embryogenesis represents promising compounds control.