Melatonin-Pretreated Mesenchymal Stem Cells Improved Cognition in a Diabetic Murine Model

• DOI: 10.3389/fphys.2021.628107
• Publication Date: 2021-03-18T06:33:37Z
• Authors: Shaimaa Nasr Amin; Nivin Sharawy; Nashwa El Tablawy; Dalia Azmy Elberry; Mira Farouk Youssef; Ebtehal Gamal Abd...; Laila Ahmed Rashed; Sherif Sabry Hassan
• Keywords: Gap-43 protein

Diabetes mellitus (DM) is a multisystem endocrine disorder affecting the brain. Mesenchymal stem cells (MSCs) pretreated with Melatonin have been shown to increase potency of MSCs. This work aimed compare Melatonin, cells, and on cognitive functions markers synaptic plasticity in an animal model type I diabetes (TIDM). Thirty-six rats represented model; six for isolation MSCs 30 were divided into five groups: control, TIDM, TIDM + Stem ex vivo Melatonin. Functional assessment was performed Y-maze, forced swimming test novel object recognition. Histological biochemical evaluation hippocampal Neuroligin 1, Sortilin, Brain-Derived Neurotrophic Factor (BDNF), inducible nitric oxide synthase (iNOS), toll-like receptor 2 (TLR2), Tumor necrosis factor-alpha (TNF-α), Growth Associated Protein 43 (GAP43). The group showed significant decrease Neuroligin, BDNF iNOS, TNF-α, TLR2, GAP43. or groups improvement compared diabetic but not control group. improvement, some values restored normal. Ex melatonin-treated had improved spatial working recognition memory depression, positive effects glucose homeostasis, inflammatory levels expression.