Myogenetic Oligodeoxynucleotide (myoDN) Recovers the Differentiation of Skeletal Muscle Myoblasts Deteriorated by Diabetes Mellitus

• DOI: 10.3389/fphys.2021.679152
• Publication Date: 2021-05-24T06:39:42Z
• Authors: Shunichi Nakamura; Shinichi Yonekura; Takeshi Shimosato; Tomohide Takaya
• Keywords: Myostatin; C2C12

Skeletal muscle wasting in patients with diabetes mellitus (DM) is a complication of decreased mass and strength, serious risk factor that may result mortality. Deteriorated differentiation precursor cells, called myoblasts, DM considered to be one the causes wasting. We recently developed myogenetic oligodeoxynucleotides (myoDNs), which are 18-base single-strand DNAs promote myoblast by targeting nucleolin. Herein, we report applicability myoDN, iSN04, myoblasts isolated from type 1 2 DM. Myogenesis was exacerbated concordantly delayed shift myogenic transcription induction interleukins. Analogous phenotypes were reproduced healthy cultured excessive glucose or palmitic acid, mimicking hyperglycemia hyperlipidemia. iSN04 treatment recovered deteriorated plural downregulating myostatin interleukin-8 (IL-8). also ameliorated impaired induced acid. These results demonstrate myoDNs can directly facilitate patients, making them novel candidates for nucleic acid drugs treat