The Response of the Estrogen-Related Receptor to 20-Hydroxyecdysone in Bombyx mori: Insight Into the Function of Estrogen-Related Receptor in Insect 20-Hydroxyecdysone Signaling Pathway

• DOI: 10.3389/fphys.2021.785637
• Publication Date: 2022-01-18T06:08:28Z
• Authors: Jinxin Wu; Guanwang Shen; Die Liu; Haoran Xu; Mengyao Jiao; Yungui Zhang; Ying Lin; Ping Zhao
• Keywords: 20-Hydroxyecdysone; Ecdysone receptor; Orphan receptor; Bombyx

Estrogen-related receptor (ERR) is an orphan nuclear that was first discovered in animals, and play important role metabolism, development, reproduction. Despite extensive research on the function of ERR, its transcriptional regulation mechanism remains unclear. In this study, we obtained upstream region Bombyx mori ERR (BmERR) confirmed promoter activity region. Interestingly, found 10 50 nM 20-hydroxyecdysone (20E) up-regulated BmERR promoter. addition, eight putative ecdysone response elements (EcREs) were predicted sequence BmERR. Based their positions, truncated into different fragments. Finally, EcRE-like (5'-AGTGCAGTAAACTGT-3') identified. Electrophoretic mobility shift assay (EMSA) cell transfection experiments motif specifically binds to complex formed between (BmEcR) ultraspiracle (BmUSP), a key 20E signaling pathway. Interference or BmEcR mRNA embryonic cells significantly affected expression BmUSP. Overall, these results suggested EcRE element identified from BmERR, will help understanding detailed regulatory insects.