Red Blood Cell Abnormalities as the Mirror of SARS-CoV-2 Disease Severity: A Pilot Study

• DOI: 10.3389/fphys.2021.825055
• Publication Date: 2023-04-13T09:40:23Z
• Authors: Anthi Bouchla; Anastasios G. Kri...; Hara T. Georgatzakou; Sotirios P. Fortis; Thomas P. Thomopo...; Leoni Lekkakou; Konstantinos Mark...; Dimitrios Gkotzias; Aikaterini Panagi...; Effie G. Papageor...; Abraham Pouliakis; Konstantinos E. S...; Sotirios G. Papag...; Vasiliki Pappa; Serena Valsami
• Keywords: SARS-CoV-2; Physiology; COVID-19; QP1-981; disease severity; chest infiltration; red blood cell; hemolysis; 3. Good health

Graphical AbstractSARS-CoV-2 infection has significant effects on red blood cells that seems to be associated with disease severity in these patients.(A)Non-Intensive care unit hospitalized patients due to severe COVID-19 disease were included in this study (n= 36).(B)The control group included healthy subjects without renal or hepatic impairment, no diagnosis of anemia or myeloid malignancy, and no blood transfusion over the past 3 months (n= 18).(C)Demographic data, clinical, laboratory and chest Computed Tomography (CT) findings at time of admission were recorded. Hematological tests, standard biochemical analysis of serum components were performed both in the patient and in the control group and coagulation analysis were evaluated in the patient group. A peripheral blood smear examination was also performed in all patients and control samples.(D)Plasma free hemoglobin and red blood cell (RBC) osmotic fragility and free hemoglobin (Free-Hb) after mechanical stress (MECH) by mechanical hemolysis were assesed in both patients and controls. Intracellular (i) calcium (iCa2 +) levels, RBCs phosphatidylserine exposure (PS), RBC membrane-bound immunoglobulins G (IgGs), and activate RBC caspase-3 levels as cell clearance and apoptotic markers were measured by flow cytometry. A spectrophotometric assay was used to quantify the activity of G6PD enzyme. Intracellular RBC reactive oxidative species (iROS) were assesed in both patients and controls.(E)Elevated hemolysis markers (Free-Hb) and Lactate Dehydrogenase (LDH) were observed in patients compared to the control group. Patients’ RBCs were more vulnerable to mechanical stress, and exhibited significantly elevated apoptotic markers (iCa2 + and RBC-PS+). Erythrocyte iROS levels were significantly lower in the patients’ group compared to healthy controls. This finding possibly correlates with the increased G6PDH activity levels in this group, suggesting a compensatory mechanism against the virus.(F)Patients’ D-Dimers correlated with RBC-PS +, implying a possible contribution of the erythrocytes in the thrombotic diathesis associated with the SARS-CoV-2 disease.(G)Free-Hb levels correlated with the extend of pulmonary infiltrates on chest CT in COVID-19 patients. RBC-IgGs+ correlated with the severity of pulmonary CT imaging features as well as the abnormality of lung function, expressed by the PO2/FiO 2 ratio. This finding suggests that hypoxia may lead to RBC senescence.