Different responses of skeletal muscles to femoral artery ligation-induced ischemia identified in BABL/c and C57BL/6 mice

• DOI: 10.3389/fphys.2022.1014744
• Publication Date: 2022-09-16T08:03:51Z
• Authors: Huiyin Tu; Junliang Qian; Dongze Zhang; Aaron N. Barksdale; Michael C. Wadman; Iraklis I. Pipinos; Yu-Long Li
• Keywords: Femoral nerve; Gastrocnemius muscle

Peripheral arterial disease (PAD) is a common circulatory problem in lower extremities, and the murine ischemic model used to reproduce human PAD. To compare strain differences of skeletal muscle responses ischemia, left femoral artery was blocked by ligation reduce blood flow limb BALB/c C57BL/6 mice. After 6 weeks ligation, functional morphological changes gastrocnemius were evaluated. mice displayed serious muscular dystrophy, including smaller myofibers (524.3 ± 66 µM2), accumulation adipose-liked tissue (17.8 0.9%), fibrosis (6.0 0.5%), compared (1,328.3 76.3 µM2, 0.27 0.09%, 1.56 0.06%, respectively; p < 0.05). About neuromuscular junctions (NMJs) muscle, induced more damage than that mice, demonstrated fragment number nicotinic acetylcholine receptor (nAChR) clusters (8.8 1.3 vs. 2.5 0.7 0.05) amplitude sciatic nerve stimulated-endplate potentials (EPPs) (9.29 1.34 mV 20.28 1.42 More importantly, significantly weakened nerve-stimulated contraction whereas it didn't alter These results suggest useful animal develop new therapeutic approaches improve structure function PAD, although mechanisms about ischemia are unclear.