Chronic sleep loss disrupts rhythmic gene expression in Drosophila

• DOI: 10.3389/fphys.2022.1048751
• Publication Date: 2022-11-18T06:59:55Z
• Authors: Zikun Wang; Samantha Lincoln; Andrew D. Nguyen; Wanhe Li; Michael W. Young
• Keywords: Sleep; Sleep loss

Genome-wide profiling of rhythmic gene expression has offered new avenues for studying the contribution circadian clock to diverse biological processes. Sleep been considered one most important physiological processes that are regulated by clock, however, effects chronic sleep loss on remain poorly understood. In present study, we exploited Drosophila mutants insomniac1 (inc1 ) and wide awakeD2 (wakeD2 as models loss. We profiled transcriptomes head tissues collected from 4-week-old wild type flies, inc1 wakeD2 at timepoints around clock. Analysis oscillation revealed a substantial rhythmicity in compared with affected genes common both mutants. The disruption was not due changes average levels. also identified subset whose shared among animals old suggesting aging chronic, sleep-loss-induced oscillation.