Ptch2 is a Potential Regulator of Mesenchymal Stem Cells

• DOI: 10.3389/fphys.2022.877565
• Publication Date: 2022-04-28T11:42:55Z
• Authors: Emma Juuri; Pauli Tikka; Andrii Domanskyi; Ian Corfe; Wataru Morita; Peter J. Mckinnon; Nela Jandova; Anamaria Balic
• Keywords: 0301 basic medicine; ORIGIN; Gli2; Physiology; Gli1; NICHE; 610 Medicine & health; 1314 Physiology; Ptch2; MOUSE; SHH; Hedgehog pathway; 10182 Institute of Oral Biology; 03 medical and health sciences; 2737 Physiology (medical); Ptch1; stem cells; GROWTH; QP1-981; PATCHED2; PERIPHERAL-NERVES; SKIN; Biochemistry, cell and molecular biology

Ptch receptors 1 and 2 mediate Hedgehog signaling pivotal for organ development homeostasis. In contrast to embryonic lethal Ptch1 −/− phenotype, Ptch2 mice display no effect on gross phenotype. this brief report, we provide evidence of changes in the putative incisor mesenchymal stem cell (MSC) niches that contribute accelerated growth, as well intriguing bones skin which suggest a role regulation MSCs their regenerative potential. We employed histological, immunostaining, computed tomography (µCT) analyses analyze morphological differences between wild-type incisors, long bones, skins. vitro CFU differentiation assays were used demonstrate MSC content potential bone marrow stromal cells. Wound healing assay was performed vivo 8-week-old assess wound closure. Loss causes increases number continuously growing incisor, associated with increased vascularization observed tooth mesenchyme neurovascular bundle. Increased length volume is linked augmented marrow. Dynamic thickness relate compartment impact closure The effects abrogation postnatal crucial